论文部分内容阅读
目的研究贵州地区人类血小板抗原(HPA1～6、15)基因多态性与急性心肌梗死(acute myocardial infarc-tion,AMI)的相关性。方法选择AMI患者86例(61.05±10.27岁),符合1979年WHO诊断标准,并行冠脉造影证实;对照组85例(60.74±6.88岁),排除心脑血管疾病及血栓性疾病。采用序列特异性引物-聚合酶链反应(SSP-PCR)技术扩增目的基因,进行HPA基因分型,回归分析其在AMI中的危险因素。结果①与对照组比较,AMI组HPA2基因多态性差异有统计学意义(P<0.01,OR=34.169,95%CI 14.553～80.221),而HPA1、3～6、15基因多态性差异无统计学意义(P>0.05);②影响AMI发病的多因素回归分析显示:与对照组比较,AMI组中HPA1ab基因型差异无统计学意义(P>0.05),但其在AMI发病的危险度比HPA1aa纯合子增加了4.256倍;HPA2aa基因型和HPA2b等位基因差异有统计学意义(P<0.01,OR=0.148,95%CI 0.071～0.307;P<0.01,OR=6.221,95%CI 3.201～12.091)。结论①HPA1ab杂合子患AMI的危险度较HPA1aa纯合子增加;②HPA2aa纯合子可能是AMI发病的保护因子,而HPA2b等位基因可能是AMI发病的独立遗传性危险因素。
Objective To study the association between polymorphisms of human platelet antigen (HPA1 ~ 6,15) and acute myocardial infarcion (AMI) in Guizhou Province. Methods Eighty-six AMI patients (61.05 ± 10.27 years) were selected, which were in line with the 1979 WHO diagnostic criteria and confirmed by coronary angiography. The control group, 85 cases (60.74 ± 6.88 years), excluded cardiovascular and thrombotic diseases. The target gene was amplified by sequence-specific primer-polymerase chain reaction (SSP-PCR) and genotyped by HPA. The risk factors of AMI were analyzed by regression analysis. Results ① Compared with the control group, the polymorphism of HPA2 gene in AMI group was significantly different (P <0.01, OR = 34.169, 95% CI 14.553 ~ 80.221), while the differences of HPA1, 3 ~ 6 and 15 gene polymorphisms (P> 0.05). (2) Multivariate regression analysis showed that there was no significant difference in genotypes of HPA1ab among AMI patients (P> 0.05), but the risk of AMI (P <0.01, OR = 0.148, 95% CI 0.071 ~ 0.307; P <0.01, OR = 6.221, 95% CI 3.201, respectively) ~ 12.091). Conclusion ①The risk of AMI in HPA1ab heterozygote is higher than that of HPA1aa homozygote; ②HPA2aa homozygote may be the protective factor of AMI, and HPA2b allele may be the independent hereditary risk factor of AMI.