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目的:探讨云南汉族人群活化性受体NKG2D基因单核苷酸多态性(SNP)与非小细胞肺癌的相关性。方法:选择云南汉族非小细胞肺癌患者467例(肺癌Ⅰ+Ⅱ期组151例,肺癌Ⅲ+Ⅳ期组316例)和健康对照384例(对照组),采用Taq Man探针基因分型方法对NKG2D基因的2个SNPs(rs2255336和rs2617160)位点进行基因分型,研究其等位基因、基因型及所构建的单倍型在肺癌Ⅰ+Ⅱ期组、Ⅲ+Ⅳ期及对照组人群中的分布差异。结果:rs2255336和rs2617160位点的等位基因及基因型在肺癌Ⅰ+Ⅱ期组、肺癌Ⅲ+Ⅳ期组和对照组中的分布频率的差异无统计学意义(P>0.05);遗传模式分析显示,rs2255336和rs2617160在Ⅰ+Ⅱ期肺癌组和Ⅲ+Ⅳ期肺癌组最优的遗传模式均为Recessive模式,其中CC-CT基因型相对于TT基因型可能是肺癌发展的保护性因素(P=0.040,OR=0.26,95%CI:0.06-1.17);rs2617160位点TT-AT基因型相对于AA基因型可能是肺癌发展的保护性因素(P=0.045,OR=0.60,95%CI:0.36-1.00)。结论:NKG2D基因rs2255336和rs2617160 SNPs位点可能与云南汉族人群非小细胞肺癌的发展有关。
Objective: To investigate the association between NKG2D gene SNP and non-small cell lung cancer in Yunnan Han population. Methods: A total of 467 non-small cell lung cancer patients (151 in lung cancer group Ⅰ + Ⅱ and 316 in lung cancer group Ⅲ + Ⅳ) and 384 healthy controls (control group) were enrolled in this study. Genotyping by Taq Man probe The genotypes of two SNPs (rs2255336 and rs2617160) in NKG2D gene were genotyped and their alleles, genotypes and haplotypes were studied in patients with stage Ⅰ + Ⅱ, Ⅲ + Ⅳ, and control groups In the distribution of differences. Results: The frequencies of alleles and genotypes of rs2255336 and rs2617160 loci in lung cancer group Ⅰ + Ⅱ, lung cancer group Ⅲ + Ⅳ and control group were not significantly different (P> 0.05). The genetic pattern analysis The results showed that the optimal genetic patterns of rs2255336 and rs2617160 in stage I + II lung cancer and stage III + IV lung cancer were Recessive pattern, in which CC-CT genotype relative to TT genotype may be a protective factor for the development of lung cancer (P = 0.040, OR = 0.26, 95% CI: 0.06-1.17). TT-AT genotype rs2617160 relative to AA genotype may be a protective factor in the development of lung cancer (P = 0.045, OR = 0.60, 95% CI: 0.36-1.00). Conclusion: The SNPs of rs2255336 and rs2617160 in NKG2D gene may be related to the development of non-small cell lung cancer in Yunnan Han population.