Expression of Human Sperm Mem brane Protein in theRecom binantSalm onella Typhim urium Vaccine

来源 :Reproduction & Contraception | 被引量 : 0次 | 上传用户:duminzl
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
A 550 bp cDNA fragment of HSD I coding for an extracellular domain of human sperm membrane protein (hSMP 1) was ligated with an Adapter containing the universal stop codon, and the ligated fragment cDNA was then cloned into the MAS of pUC19. The desired plasmid with correct open reading frame was obtained, and was cut with EcoR I.The insert was purified and then cloned into the two asd + Salmonella expression vectors (the low copy number plasmid pYA292 and the high copy number plasmid pYA3137). The recombinant plasmid containing the insert with the correct orientation was selected by restriction enzyme digestion analysis. The recombinant plasmids were transferred into the non pathogenic Salmonella typhimurium χ4550, which was deletion of the Δcya, Δcrp and Δasd genes. Western blot analysis of the whole cell lysate of the two recombinants of S. typhimurium showed a predominant protein band at 21 KD, which reacted with the anti hSMP 1 antiserum. The result indicated that two recombinants of S. typhimurium containing the 550 bp cDNA of HSD I were constructed and the characteristics of their growth in vitro were determined. They may be used as new potential mucosal immunization antifertility. A 550 bp cDNA fragment of HSD I coding for an extracellular domain of human sperm membrane protein (hSMP 1) was ligated with an Adapter containing the universal stop codon, and the ligated fragment cDNA was then cloned into MAS of pUC19. The desired plasmid with correct open reading frame was obtained, and was cut with EcoR I. The insert was purified and then cloned into the two asd + Salmonella expression vectors (the low copy number plasmid pYA292 and the high copy number plasmid pYA3137). the insert with the correct orientation was selected by restriction enzyme digestion analysis. The recombinant plasmids were transferred into the non pathogenic Salmonella typhimurium χ4550, which was deletion of the Δcya, Δcrp and Δasd genes. Western blot analysis of the whole cell lysate of the two recombinants of S. typhimurium showed a predominant protein band at 21 KD, which reacted with the anti hSMP 1 antiserum. The result indic ated that two recombinants of S. typhimurium containing the 550 bp cDNA of HSD I were constructed and the characteristics of their growth in vitro were determined. They may be used as new potential mucosal immunization antifertility.
其他文献
对于科研事业单位而言,财务内部控制工作的有效实施不仅可以促进其岗位责任制的构建,还可以对单位内部工作氛围进行优化,使财务内部控制工作的顺利高效实施更有保障。但是目前很
村镇土地利用中存在空心村、非法占用等一系列问题,土地利用的监测、评价、预警是保证土地资源合理配置的技术依据.本文首先针对土地利用预警指标体系构建、指标值获取技术、
会议
我国是一个水资源严重缺乏,水旱灾害频繁的国家.已被联合国列为13 个贫水国家之一.农业是我国的用水大户,当前,对农业进行精确化灌溉主要采用湿度检测及气象观测站方式进行反
会议
伴随我国房价的不断上涨,在房地产领域中各项调控政策也陆续出台了,使得我国房地产企业的总体业绩情况产生了较大的波动。在此背景之下,为更好的下降企业的税收成本,则应注重进行
本文提出一种模拟客户端HTTP请求定向获取web 文本并进一步加工以抓取所需数据的方法,以第二次提取数据的形式实施数据的重复利用.利用一个可以制定抓取页面内容并制定抓取页
会议
物联网产业化发展是基于传感网络、超级计算、云计算和数据存储挖掘等相关信息技术产业链的发展,规划建设物联网信息系统,需要将关键技术和信息资源管理作为基础.信息资源是
会议
当前,国有企业实现了较快发展,为我国的经济发展做出贡献。同时,我国大多数企业市场化改革不断推进,国有企业在这个过程中面临着更多的风险。因此,本文主要对国有企业财务管理风险
本文主要以全面预算管理为核心的企业内部控制为论据,通过阐述全面预算管理与企业内部控制的关系,即,目标一致,相互促进共同发展,全面预算管理风险的控制等。基于此,提出了几点有针
建筑企业执行科学的全面预算以及绩效考核模式,能提升其对于企业稳定安全发展以及盈利模式优化效果。能做大搜在建筑企业的发展过程中,将基本的管理优势发挥出来,实现在建筑企业
文章基于对企业两金概念的介绍,分析“两金”管控工作对于企业发展的重要作用,结合综合性建筑施工企业中的“两金”管控情况和成因,提出了相应的两金管控措施,以供参考。