采用栽培大麦和普通小麦属间四个杂交组合,进行杂种幼胚离体培养,并以杂种胚和杂种F_1的幼穗为外植体,通过组织培养无性繁殖,相继获得大量杂种F_1的再生植株。杂种F_1无性系再生植株体细胞染色体数,除具大小麦杂种F_1预期的2n=28外,还有非整倍体2n=26和27的单株,其花粉母细胞(PMC)减数分裂中期MI时,配对二价体染色体数较原大小麦杂种F_1的偏多。杂种F_1无性系有一再生植株,在穗部形态上呈现出大麦三联小穗的特征,这些再生植株的回交后代有雄蕊发育正常株。再生植株的回交后代,在穗型、抽穗期和株高方面都表现出较大的变异。 Four hybrid combinations of cultivated barley and common wheat were used to carry out in vitro culture of hybrid embryos in vitro. The hybrid embryos and F1 hybrids were used as explants, and a large number of regenerated plants of F1 hybrids were obtained through tissue culture . In addition to the 2n = 28 expected from the F1 hybrids of F1 and F1 hybrids, there were 2n = 26 and 27 aneuploidy individuals with pollen mother cells (PMCs) in metaphase meiosis MI, the number of paired bivalent chromosomes was larger than the original wheat F1 hybrids. The hybrid F_1 clones had a regenerated plant which showed the characteristic of the spikelet triplet spikelets in the panicle morphology. The backcross generations of these regenerated plants had the normal stamen development. Backcross progenies of regenerated plants showed larger variation in panicle type, heading date and plant height.