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应用Ｓ—Ｐ免疫组织化学方法研究６２例（男性５１例，女性１１例，年龄３４～６８岁，平均年龄５３．７岁）肺癌组织中Ｐ１６蛋白和Ｐ５３蛋白的表达情况，并进行增殖细胞核抗原（ＰＣＮＡ）检测，计算细胞增殖指数（Ｐｒｏｌｉｆｅｒａｔｉｏｎｉｎｄｅｘ，ＰＩ）。６２例肺癌组织中Ｐ１６蛋白和Ｐ５３蛋白阳性率分别为５８．１％和５９．７％。腺癌Ｐ１６蛋白的阳性率明显高于小细胞癌（Ｐ＜０．０５）；淋巴结转移阳性组Ｐ１６蛋白的表达显著低于阴性组（Ｐ＜０．０５）；ＰＩ分级为Ⅱ级的Ｐ１６蛋白表达显著高于Ⅳ级（Ｐ＜０．０５）。不同组织类型肺癌中Ｐ５３蛋白的表达未见明显差异，淋巴结转移阳性组Ｐ５３蛋白的表达高于阴性组（Ｐ〈０．０１）；不同ＰＩ分级中Ｐ５３蛋白的表达，Ⅳ级明显高于Ⅰ级（Ｐ〈０．０５）和Ⅱ级（Ｐ〈０．０５），Ⅲ级明显高于Ⅰ级（Ｐ〈０．０５）和Ⅱ级（Ｐ〈０．０５）。Ｐ１６蛋白低表达和Ｐ５３蛋白过表达之间未见明显相关。揭示Ｐ１６蛋白低表达和Ｐ５３蛋白过表达均有促进肺癌细胞增殖的作用，Ｐ１６蛋白的表达与肺癌的细胞分化有关，Ｐ５３蛋白过表达对肺癌细胞的转移起重要作用。抑癌基因Ｐ５３对ＭＴＳ１／Ｐ１６基因无明显调控作用，检测Ｐ５３蛋白表达?
S-P immunohistochemical method was used to study the expression of P16 protein and P53 protein in 62 cases (51 males, 11 females, aged 34-68 years, mean age 53.7 years) and performed proliferating cell nuclear antigen. (PCNA) Assay, Calculate Cell Proliferation Index (PI). The positive rates of P16 protein and P53 protein in 62 lung cancer tissues were 58.1% and 59.7%, respectively. The positive rate of P16 protein in adenocarcinoma was significantly higher than that in small cell carcinoma (P<0.05); the expression of P16 protein in lymph node metastasis positive group was significantly lower than that in negative group (P<0.05); PI grade was grade II P16 protein The expression was significantly higher than that of grade IV (P<0.05). There was no significant difference in the expression of P53 protein in different tissue types of lung cancer. The expression of P53 protein in lymph node metastasis positive group was higher than that in negative group (P<0.01). The expression of P53 protein in different PI grades was significantly higher than that in grade I. (P<0.05) and grade II (P<0.05), grade III was significantly higher than grade I (P<0.05) and grade II (P<0.05). There was no significant correlation between low expression of P16 protein and overexpression of P53 protein. It was revealed that low expression of P16 protein and overexpression of P53 protein all promote the proliferation of lung cancer cells. The expression of P16 protein is related to cell differentiation of lung cancer. Overexpression of P53 protein plays an important role in the metastasis of lung cancer cells. The tumor suppressor gene P53 had no obvious regulation on MTS1/P16 gene. The expression of P53 protein was detected.