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目的:探讨低分子量硫酸软骨素(CS)在兔软骨修复过程中对于IL-1β、TNF-α和TGF-β及血液中钙磷含量的影响。方法:36只成年新西兰大白兔,随机分成6组,分别为对照组、模型组、低分子量CS低剂量组、低分子量CS高剂量组、高分子量CS低剂量组、高分子量CS高剂量组,每组6只。通过在实验兔股骨髁关节面部位,钻出直径3mm,深度3mm的缺孔,造成其关节软骨的缺损。术后次日给予药物进行灌胃,每日1次,5周后取材。采用酶联免疫法测定关节液中IL-1β、TNF-α和TGF-β的含量,同时用全自动生化分析仪及其配套的试剂盒来测定兔血清中钙,磷含量。结果:低分子量CS和高分子量CS都能够减少关节液中炎性因子的含量和增加TGF-β的含量,且与模型组相比具有统计学意义(P<0.05);同时低分子量CS与高分子量CS相比效果较好(P<0.05);而高低剂量之间无统计学意义(P>0.05)。与模型组相比,给予CS的组别,其血清中的钙磷含量相对较少(P<0.05),但都高于对照组。结论:高、低分子量的CS都可以增加TGF-β的含量,降低IL-1β、TNF-α和血清中钙、磷的含量。而对于软骨修复,这可能是通过对上述因子的影响,从而产生积极的作用。
Objective: To investigate the effects of low molecular weight chondroitin sulfate (CS) on IL-1β, TNF-α, TGF-β and blood calcium and phosphorus during the repair of rabbit cartilage. Methods: Thirty-six adult New Zealand white rabbits were randomly divided into 6 groups: control group, model group, low-dose CS low dose group, low-dose CS high dose group, high-molecular weight CS low dose group, high- 6 per group Through the experimental rabbit femoral condyle articular surface of the site, the diameter of 3mm, the depth of 3mm hole, resulting in the loss of its articular cartilage. The day after surgery to give drugs for gavage, 1 day, 5 weeks after drawing. The levels of IL-1β, TNF-α and TGF-β in synovial fluid were determined by enzyme-linked immunosorbent assay. Meanwhile, the content of calcium and phosphorus in rabbit serum was determined by automatic biochemical analyzer and its kit. Results: Both low molecular weight CS and high molecular weight CS could decrease the content of inflammatory cytokines and increase the content of TGF-β in the synovial fluid, which was statistically significant compared with the model group (P <0.05) The molecular weight of CS was better (P <0.05), but there was no significant difference between high and low dose (P> 0.05). Compared with the model group, the serum levels of calcium and phosphorus in the CS group were less (P <0.05), but higher than those in the control group. CONCLUSION: Both high and low molecular weight CS can increase the content of TGF-β and decrease the levels of IL-1β, TNF-α and serum calcium and phosphorus. For cartilage repair, this may be through the impact of these factors, which have a positive effect.