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目的探讨间充质干细胞(MSCs)与造血干细胞(HSCs)共同移植对造血重建的影响。方法将扩增的Balb/c小鼠骨髓MSCs与骨髓共同经尾静脉输入经致死剂量照射的同系小鼠体内(联合输注组),并设单输骨髓细胞的HSCs组、单输间充质干细胞的MSCs组以及输培养基的对照组,每隔5d计数白细胞,计算动物死亡率。将乙酰乙酸碳氧荧光素标记的人间充质干细胞输入SCID小鼠尾静脉,24h后取肺、心、肝、脾、肾和小肠组织做冰冻切片;取骨髓、肝脏及脾脏,制成单细胞悬液,涂片,在荧光显微镜下观察计数;用逆转录聚合酶链反应测定鼠骨髓中人特异性β2-微球蛋白。结果细胞输注后,联合移植组的白细胞恢复快,12d左右恢复正常,死亡率(3/11)低于HSCs组(5/10)、MSCs组(4/4)、输培养基的对照组(11/11)。人MSCs输入SCID小鼠后24h,其在体内的分布依次为肺、肾、脾、肝,小肠及心脏组织中几乎无阳性细胞,骨髓中有极少MSCs。结论MSCs与造血干细胞共输能促进造血恢复。
Objective To investigate the effects of co-transplantation of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) on hematopoietic reconstitution. Methods The expanded Balb / c mice bone marrow MSCs were co-transconjugated into the tail vein of mice and injected into the lethal dose-irradiated syngeneic mice (combination group), and single-myeloid bone marrow HSCs group, The MSCs group of stem cells and the control group of the culture medium were counted for white blood cells every 5 days to calculate the animal mortality rate. Fetal mesenchymal stem cells labeled with acetoacetate were transfected into the tail vein of SCID mice and frozen sections were taken from lung, heart, liver, spleen, kidney and small intestine after 24 hours. Bone marrow, liver and spleen were removed to obtain single cells Suspension, smear, observed under a fluorescent microscope count; reverse transcription polymerase chain reaction of human bone marrow-specific β2-microglobulin. Results After the cells were transfused, the leukocytes recovered rapidly in the combined transplantation group and returned to normal after 12 days. The mortality rate (3/11) was lower than that in HSCs group (5/10), MSCs group (4/4) (11/11). Human MSCs into SCID mice after 24h, its distribution in the body followed by lung, kidney, spleen, liver, small intestine and heart tissue almost no positive cells, bone marrow MSCs rarely. Conclusion The co-administration of MSCs and hematopoietic stem cells can promote the recovery of hematopoiesis.