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目的建立气相色谱法测定山蜡梅叶中桉油精和芳樟醇的方法。方法气相色谱法,DB-WAX石英毛细管柱(30.0 m×250μm,0.25μm),FID检测器,进样口温度:230℃,检测口温度250℃,体积流量1 mL/min,分流:20∶1,程序升温:起始温度60℃,保持5 min,以25℃/min升至200℃,保持2 min。结果桉油精在0.015 7～0.393 6 mg/mL与峰面积比值(桉油精/环己酮)呈良好的线性关系,回归方程为Y=13.659X+0.066 5,r=0.999 8,平均回收率为98.29%,RSD为1.53%;芳樟醇在0.009 5～0.236 6 mg/mL与峰面积比值(芳樟醇/环己酮)呈良好的线性关系,回归方程为Y=13.526X+0.016 6,r=0.999 6,平均回收率为98.55%,RSD为1.96%。结论采用气相色谱法对山蜡梅叶中桉油精和芳樟醇进行定量测定,方法简便、快速、准确、重现性好。
OBJECTIVE To establish a method for the determination of eucalyptol and linalool in the leaves of Pseudostellaceae Japonica by gas chromatography. Methods Gas chromatography, DB-WAX quartz capillary column (30.0 m × 250μm, 0.25μm), FID detector, inlet temperature: 230 ℃, detection port temperature of 250 ℃, volume flow rate of 1 mL / min, 1, the program temperature: the starting temperature of 60 ℃, for 5 min, with 25 ℃ / min rose to 200 ℃, hold 2 min. Results Eucalyptol showed a good linear relationship between the peak area ratio (eucalyptol / cyclohexanone) and 0.015 7 ~ 0.393 6 mg / mL, the regression equation was Y = 13.659X + 0.066 5, r = 0.999 8, with an average recovery The rate was 98.29% and the RSD was 1.53%. Linalool had a good linear relationship with the peak area ratio (linalool / cyclohexanone) at 0.009 5 ~ 0.236 6 mg / mL, the regression equation was Y = 13.526X + 0.016 6, r = 0.999 6, the average recovery was 98.55%, RSD was 1.96%. Conclusion The method for the quantitative determination of eucalyptol and linalool in P. chrysanthum by gas chromatography is simple, rapid, accurate and reproducible.