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趋化性是多形核白细胞(PMN_s)的主要功能之一,可使它们局部定位和移动到发炎部位。趋化性包含的主要标志是C5a,它是一种由C5a受体识别的补体级联(complement cascade)产物,fMLP是一种通过fMLP受体识别的许多甲酰化细菌肽类的小分子肽原型;LTB_4是被各自不同的细胞,特别是PMN_s所分泌的一种白三烯(leukotriene),和由单核和多形核吞噬细胞产生的血小板活性因子(PAF)。某些抗生素能显示出干扰PMN_s的移动作用。研究PMN_s移动的两个最通用的方法是Nelson等的琼脂糖法和Boyden Chamber法,后法主要取决于PMNs的变形性,且该法具有很高的变异性。
Chemotaxis is one of the major functions of polymorphonuclear leukocytes (PMNs), allowing them to localize and move to the site of inflammation. The major hallmark of chemotaxis inclusion is C5a, a complement cascade product recognized by the C5a receptor, a small molecule peptide of many formylated bacterial peptides recognized by the fMLP receptor Prototype; LTB_4 is a leukotriene secreted by different cells, especially PMN_s, and platelet-activating factor (PAF) produced by mononuclear and polymorphonuclear phagocytes. Some antibiotics can show a disruptive effect on PMN_s. The two most common methods for studying the movement of PMN_s are the Nelson et al. Agarose method and the Boyden Chamber method. The latter method mainly depends on the deformability of PMNs and the method has very high variability.