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从出现口咽腔溃烂症的中华鳖 ( Trionyx sinensis)脾脏内分离出两种菌株 990 72 9、990 80 4 ,用这两菌株人工感染 10 0～ 150 g健康鳖 ,发病率和死亡率为 10 0 %。经细菌形态学观察 ,培养特征和生理生化反应测定 ,鉴定 990 72 9为维罗纳气单胞菌 ( Aeromonas veronii) ,990 80 4为布氏柠檬酸杆菌 ( Citrobacter braakii)。这两种菌都会引起鳖口咽腔溃烂 ,肝、肾、脾和肠明显病变。2 5种药物的药敏试验结果表明 :维罗纳气单胞菌对壮观霉素、头孢三嗪和妥布霉素敏感 ;布氏柠檬酸杆菌对诺氟沙星、头孢三嗪和环丙沙星敏感。
Two strains of 990 729, 9090 80 4 were isolated from the spleen of Trionyx sinensis with oropharyngeal cavity ulceration. The two strains were used to artificially infect 10 0-150 g of healthy turtle with a morbidity and mortality of 10 0%. Bacterial morphology, culture characteristics and physiological and biochemical reactions were determined. The results showed that 990 729 was Aeromonas veronii and 990 80 4 was Citrobacter braakii. These two kinds of bacteria can cause pharyngeal turtle mouth ulceration, liver, kidney, spleen and intestine obvious lesions. The results of drug susceptibility test of 5 drugs showed that Aeromonas verona was sensitive to spectinomycin, ceftriaxone and tobramycin. Citrobacter brasilse was sensitive to norfloxacin, ceftriaxone and cyclosporine Shaxing sensitive.