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目的:建立以槲皮素为内参物,同时测定窝儿七中3个活性成分(槲皮素、鬼臼毒素、山柰素)的一测多评法。方法:以窝儿七为研究对象,以槲皮素为内参物,建立鬼臼毒素、山柰素2个成分相对于槲皮素的相对校正因子。采用外标法测定窝儿七中槲皮素的含量,通过相对校正因子对其他2个成分进行定量分析,并将该方法的测定结果与外标法的测定结果相比较。结果:各成分相对校正因子重现性良好。槲皮素、鬼臼毒素、山柰素按一测多评方法与外标法测定结果无显著差异。结论:本文建立的方法经方法学验证,可为控制窝儿七的内在质量提供参考。
Objective: To establish a multi-evaluation method using quercetin as reference material and simultaneous determination of three active constituents (quercetin, podophyllotoxin and kaempferol) in Pleurotus. Methods: The study aimed to study the effects of podophyllotoxin and kaempferol on the relative correction factors of quercetin, kaempferol and quercetin. The content of quercetin in Fusanqi was determined by external standard method, and the other two components were quantitatively analyzed by relative correction factor. The results of this method were compared with those of external standard method. Results: The relative correction factor of each component has good reproducibility. Quercetin, podophyllotoxin, behen Su element by a multi-evaluation method and the external standard test results no significant difference. Conclusion: The method established in this paper is verified by methodology, which can provide reference for controlling the intrinsic quality of Infantile Seven.