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目的探讨葡萄糖转运蛋白-1(Glut-1)对检测卵巢上皮性肿瘤腹水中有/无肿瘤细胞的诊断价值。方法选取卵巢上皮性肿瘤的腹水标本30例,制成细胞沉渣蜡块,采用免疫细胞化学二步法检测Glut-1、CEA、CA125与pan-CK在肿瘤细胞中的表达,并与腹水细胞学检查及28例大网膜组织病理学检查结果进行比较。结果①30例腹水细胞学阳性、阴性与可疑病例中,Glut-1阳性率分别为83.3%(15/18)、20%(2/10)和50%(1/2),细胞学阳性与阴性病例的Glut-1阳性率差异显著(P<0.05);②28例大网膜阳性、阴性与可疑病例中,Glut-1阳性率分别为93.3%(14/15)、33.3%(4/12)和0(0/1),大网膜阳性与阴性病例的Glut-1阳性率差异显著(P<0.05);③CEA、CA125和pan-CK的阳性率在细胞学阳性病例中分别为22.2%(4/18)、88.8%(16/18)和100%(18/18),在大网膜阳性病例中分别为13.3%(2/15)、93.3%(14/15)、100%(15/15),它们在细胞学与大网膜阳性及阴性病例中的阳性率比较差异不显著(P>0.05)。结论 Glut-1是一个较有价值的卵巢上皮性肿瘤标记物,可用以辅助鉴别诊断卵巢癌腹水中的肿瘤细胞与反应性间皮细胞。
Objective To investigate the diagnostic value of glucose transporter-1 (Glut-1) in detecting ascites in ascites of ovarian epithelial tumors. Methods 30 cases of ovarian epithelial tumor ascites specimens were selected, the cell sediment wax blocks were made, the expression of Glut-1, CEA, CA125 and pan-CK in tumor cells were detected by immunocytochemical two-step method and compared with ascites cytology Check and 28 cases of omental histopathological examination results were compared. Results ① The positive rates of Glut-1 in 30 cases of ascites were 83.3% (15/18), 20% (2/10) and 50% (1/2), respectively. The positive and negative cytology The positive rates of Glut-1 in the 28 cases of omental positive, negative and suspicious cases were 93.3% (14/15) and 33.3% (4/12), respectively And 0 (0/1) respectively. The positive rates of Glut-1 in omental positive and negative cases were significantly different (P <0.05). The positive rates of CEA, CA125 and pan-CK were 22.2% 4/18), 88.8% (16/18) and 100% (18/18) respectively, and in the omental positive cases, 13.3% (2/15), 93.3% (14/15) and 100% / 15). The positive rates of cytology and omentum in both positive and negative cases were not significantly different (P> 0.05). Conclusion Glut-1 is a valuable marker of ovarian epithelial tumor, which can be used to assist in differential diagnosis of tumor cells and reactive mesothelial cells in ascites of ovarian cancer.