蛋白质分子量是蛋白质分析研究工作中最基本的物理化学参数之一。其测定方法有渗透压法、光散射法。但是,这两种方法的灵敏度较低,测定误差较大。目前,研究工作中广为使用的测定方法是超离心法、凝胶过滤法和SDS-聚丙烯酰胺凝胶电泳法,这些测定方法均可得到较为准确的结果。但是,必须配备特殊的仪器,操作时间长。 Protein molecular weight is one of the most basic physicochemical parameters in protein analysis. The determination of osmotic pressure method, light scattering method. However, the sensitivity of these two methods is lower and the measurement error is greater. At present, the widely used determination methods in the research work are ultracentrifugation, gel filtration and SDS-polyacrylamide gel electrophoresis, and these determination methods can obtain more accurate results. However, special equipment must be provided for long operating hours.