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目的:建立同时测定淡豆豉中的大豆苷、黄豆黄苷、染料木苷、黄豆苷元、黄豆黄素、染料木素6种异黄酮类成分含量的UPLC方法。方法:采用ACQUITY UPLC~ BEH C_(18)色谱柱(50 mm×2.1 mm,1.7μm);流动相为0.1%冰乙酸-乙腈,梯度洗脱;流速0.3 mL/min;检测波长254 nm;柱温25℃;进样量10μL。结果:大豆苷、黄豆黄苷、染料木苷、黄豆苷元、黄豆黄素、染料木素分别在0.088~5.280μg/mL(r_1=0.9997)、0.092~5.520μg/mL(r_2=0.9998)、0.152~9.120μg/mL(r_3=0.9998)、0.100~6.000μg/mL(r_4=0.9999)、0.072~4.320μg/mL(r_5=0.9998)、0.048~3.840μg/mL(r_6=0.9995)范围内线性关系良好,精密度、重现性、回收率均符合要求。结论:该方法快速、简便,重复性好,适用于同时测定淡豆豉中大豆苷、黄豆黄苷、染料木苷、黄豆苷元、黄豆黄素、染料木素的含量。
OBJECTIVE: To establish an UPLC method for the simultaneous determination of six isoflavones in daidzein, including daidzin, glycitein, genistin, daidzein, glycitein and genistein. Methods: The chromatographic column (50 mm × 2.1 mm, 1.7 μm) with ACQUITY UPLC ~ BEH C 18 column was used. The mobile phase consisted of 0.1% glacial acetic acid - acetonitrile with a gradient of 0.3 mL / min. The detection wavelength was 254 nm. Column temperature 25 ℃; injection volume 10μL. Results: The concentrations of daidzin, glycoside, daidzein, daidzein and genistein were 0.088-5.280μg / mL (r_1 = 0.9997), 0.092-5.520μg / mL (r_2 = 0.9998) (R_4 = 0.9999), 0.072 ~ 4.320μg / mL (r_5 = 0.9998) and 0.048 ~ 3.840μg / mL (r_6 = 0.9995) in the range of 0.152 ~ 9.120μg / mL Good relationship, precision, reproducibility, recovery rate are in line with the requirements. Conclusion: The method is rapid, simple and reproducible. It is suitable for the simultaneous determination of daidzin, glycitein, genistin, daidzein, glycitein and genistein in