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大花君子兰是具有广泛市场价值的重要室内观赏花卉。但是,常规遗传育种周期时间长,污染率高和转化率低等原因,是君子兰遗传育种中普遍存在的问题。针对这些问题,本研究构建了以甘露糖异构酶基因(Pmi)为筛选基因并带有玉米Pl转录因子的pBI121-Pl真核表达载体,以茎尖分生组织作为受体,进行了大花君子兰遗传转化体系的探究。确定了无菌植株的最佳遗传转化方案、准确的共培养时间和温度以及最适甘露糖筛选浓度,初步证明Pl基因已成功整合到大花君子兰基因组中并获得了转基因植株,有助于君子兰的遗传育种的研究。
Grandmother Clivia is an important indoor ornamental flower with a wide market value. However, the conventional genetic breeding cycle of a long time, high pollution rates and low conversion rates are the common problems in the genetic breeding of Clivia. In response to these problems, we constructed a pBI121-P1 eukaryotic expression vector with mannose isomerase gene (Pmi) as the screening gene and maize P1 transcription factor. The apical meristem was used as the receptor Genetic Transformation of Flowering Clivia. The best genetic transformation program of sterile plants, accurate co-culture time and temperature, and optimal mannose screening concentration were determined. The results showed that P1 gene was successfully integrated into the genome of Clivia magnolia and obtained transgenic plants, The study of genetic breeding.