论文部分内容阅读
目的:探讨辛热药附子、肉桂、仙茅对正常大鼠药物代谢酶细胞色素P4503A(CYP3A)和谷胱甘肽-S转移酶(GST)活性的影响,为辛热药性的科学阐释及临床安全合理用药提供依据。方法:SD雄性大鼠随机分为4组,空白组灌服自来水,其他各组分别灌胃附子(12g.kg-1.d-1)、肉桂(30g.kg-1.d-1)、仙茅(20g.kg-1.d-1)水煎液,连续7d后,检测肝、小肠微粒体CYP3A活性及肝微粒体和血浆GST活性。结果:与正常组比,附子、肉桂、仙茅组大鼠肝微粒体CYP3A和GST活性显著升高(P<0.01),而小肠微粒体CYP3A活性变化不明显,血浆GST活性有下降趋势。结论:附子、肉桂、仙茅能明显升高大鼠肝脏CYP3A和GST活性,可能是其辛热药性表达的生物学基础之一。
Objective: To investigate the effects of aconite, cinnamon and Curculigoside on cytochrome P4503A (CYP3A) and glutathione S-transferase (GST) activities in normal rats, and to provide a scientific explanation of the syndactyly effect and clinics Safe and rational use of drugs to provide the basis. Methods: SD male rats were randomly divided into 4 groups. The blank group was fed with tap water. The other groups were given ganoderma lucidum (12g.kg-1.d-1), cinnamon (30g.kg-1.d-1) Curculigo (20g.kg-1.d-1) decoction, after continuous 7d, liver and small intestine microsomal CYP3A activity and liver microsomes and plasma GST activity. Results: Compared with the normal group, the activities of CYP3A and GST in the liver microsomes were significantly increased (P <0.01), while the activity of the microsomal CYP3A in the small intestine was not significantly changed, while the plasma GST activity was decreased. CONCLUSION: Aconite, cinnamon and Curculigoside can obviously increase the activity of CYP3A and GST in the liver of rats, which may be one of the biological bases of their synergistic effects.