目的　观察一种结构新颖的HCV融合抗原DNA疫苗在BALB c小鼠的免疫效果 ,探讨其用于防治丙型肝炎的可行性。方法　用重叠延伸PCR拼接编码小鼠IgGkappa链信号肽和通用型辅助性T细胞表位PADRE的DNA片段 ,PCR分别扩增HCV核心抗原基因和包膜E2抗原基因 ,将 3段基因插入真核表达载体pcDNA3.1,构成重组表达质粒pST CE2t,转染COS7细胞 ,免疫组化检测HCV抗原的表达。将pST CE2t和HCV核心抗原DNA疫苗pcDNA3.1core分别肌肉注射接种BALB c小鼠 ,检测小鼠的血清抗体、T细胞增殖和CTL反应。结果　pST CE2t可在COS7细胞内表达HCV核心抗原和E2抗原 ,接种于BALB c小鼠能有效诱导体液和细胞免疫应答 ,其中抗HCV核心抗原免疫应答的强度明显超过pcDNA3.1core ,且更趋向于TH1型免疫应答。结论　pST CE2t对于丙型肝炎的防治有潜在的应用价值 Objective To observe the immunogenicity of a novel DNA vaccine against HCV fusion antigen in BALB mice and explore the feasibility of its use in the prevention and treatment of hepatitis C virus. Methods DNA fragments of mouse IgGkappa chain signal peptide and universal helper T cell epitope PADRE were spliced ​​by overlap extension PCR. HCV core antigen gene and E2 antigen gene were amplified by PCR and inserted into eukaryotic expression vector The vector pcDNA3.1 was constructed, and the recombinant plasmid pST CE2t was constructed and transfected into COS7 cells. The expression of HCV antigen was detected by immunohistochemistry. The pST CE2t and HCV core antigen DNA vaccine pcDNA3.1core were intramuscularly inoculated BALB c mice to detect serum antibody, T cell proliferation and CTL response. Results pST CE2t expressed HCV core antigen and E2 antigen in COS7 cells. Inoculation of BALB / c mice effectively induced humoral and cellular immune responses. The anti-HCV core antigen immune response intensity was significantly higher than that of pcDNA3.1core, TH1 type immune response. Conclusion pST CE2t has potential value for the prevention and treatment of hepatitis C infection