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用Ｂｏｌｔｏｎ－Ｈｕｎｔｅｒ试剂联结法标记胆囊收缩素（ＣＣＫ８），得１２５Ｉ－ＢＨ－ＣＣＫ８，其比放射性为３．４ＴＢｑ／ｍｍｏｌ，放射化学纯度大于９６％。取其与自制的大鼠大脑皮质细胞膜进行受体放射分析，发现标记配体与大鼠大脑皮质ＣＣＫ受体的结合具有温度和时间依赖性，可饱和性，可逆性及特异性。经Ｓｃａｔｃｈａｒｄ分析获大鼠大脑皮质细胞膜ＣＣＫ受体Ｋｄ值为１．０９８ｎｍｏｌ／Ｌ，Ｂｍａｘ为１９７．５ｆｍｏｌ／ｍｇ蛋白。
Cholecystokinin (CCK8) was labeled with Bolton-Hunter reagent to obtain 125I-BH-CCK8 with specific radioactivity of 3.4TBq / mmol and radiochemical purity of greater than 96%. The radioimmunoassay results showed that the binding of labeled ligand to CCK receptor in rat cerebral cortex was temperature-dependent, time-dependent, saturable, reversible and specific. Scatchard analysis showed that the Kd value of CCK receptor in rat cerebral cortex cells was 1.098 nmol / L and the Bmax was 197.5 fmol / mg protein.