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目的:探讨中国北方汉族人非小细胞肺癌(NSCLC)临床症状及病理类型与mdm2基因多态性的关联性。方法:采用聚合酶链反应(PCR)和限制性内切酶片段长度多态性(RFLP)方法检测88例NSCLC患者的mdm2基因型(GG,AG和AA),并通过Pearson卡方检验及Woolf方法计算相对风险度(OR)及95%可信区间(95%CI),验证mdm2基因多态性与各临床症状及病理类型之间的关联性。结果:mdm2基因单核苷核多态性(SNP)(rs1196337:A/G碱基互换)的AA基因型与NSCLC的咳嗽症状呈显著相关(P<0.05),AA基因型频数分布高于GG和AG基因型。结论:mdm2基因多态性与NSCLC的咳嗽症状高度相关。
Objective: To investigate the association of clinical symptoms and pathological types with mdm2 gene polymorphisms in Han Chinese non-small cell lung cancer (NSCLC) in northern China. Methods: The mdm2 genotypes (GG, AG and AA) of 88 patients with NSCLC were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Pearson chi square test and Woolf Methods The relative risk (OR) and 95% confidence interval (95% CI) were calculated to verify the relationship between mdm2 gene polymorphism and clinical symptoms and pathological types. Results: AA genotypes of mdm2 SNP (rs1196337: A / G base exchange) were significantly associated with cough symptoms in NSCLC (P <0.05). The frequency distribution of AA genotype was higher than GG and AG genotypes. Conclusion: The mdm2 gene polymorphism is highly correlated with cough symptoms in NSCLC.