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目的 验证新鲜人脑胶质瘤细胞是否能自发分泌和表达白细胞介素 6 (interleukin 6 ,IL 6 )并进一步明确IL 6在人脑胶质瘤发生及发展中的作用。方法 采用手术中直接获得的人脑胶质瘤组织 ,进行胶质瘤细胞的原代培养 ,对瘤细胞培养上清进行IL 6生物活性检测 ,然后采用逆转录聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)方法测定胶质瘤细胞中IL 6mRNA的表达 ;在确定人脑胶质瘤细胞有IL 6的分泌及表达后 ,再分别采用重组IL 6 (recombinantIL 6 ,rIL 6 )及IL 6单克隆抗体 (monoclonalantibody ,McAb)处理脑胶质瘤细胞。结果 原代培养的人脑胶质瘤细胞在无任何刺激的情况下可自发分泌IL 6 ,并在一定范围内呈剂量依赖性关系 ,而正常脑胶质细胞则无IL 6的分泌。 2 0例人脑胶质瘤标本中有 17例表达IL 6mRNA ,且其表达量与脑胶质瘤的恶性程度呈正相关关系。rIL 6能刺激脑胶质瘤细胞的增殖 ,而IL 6McAb则能抑制脑胶质瘤细胞的增殖。结论 新鲜人脑胶质瘤细胞亦能自发分泌和表达IL 6 ,人脑胶质瘤中也存在IL 6的自分泌环路 ,这种自分泌环路的存在参与维持脑胶质瘤的恶性增殖
Objective To verify whether fresh human glioma cells can secrete and express interleukin 6 (IL6) spontaneously and further clarify the role of IL 6 in the development and progression of human glioma. Methods Human glioma tissues obtained directly during surgery were used to perform primary culture of glioma cells. IL 6 bioactivity was detected in tumor cell culture supernatants, and then reverse transcription polymerase chain reaction (RT) was used. The expression of IL 6 mRNA in glioma cells was determined by PCR). After determining the secretion and expression of IL 6 in human glioma cells, recombinant IL 6 (rIL 6) and IL 6 monoclonal antibodies were used. (monoclonal antibody, McAb) treatment of glioma cells. Results Primary cultured human glioma cells spontaneously secreted IL-6 without any stimulation, and showed a dose-dependent manner within a certain range, whereas normal brain glial cells did not have IL-6 secretion. Of the 20 human glioma specimens, 17 were found to express IL-6 mRNA, and their expression levels were positively correlated with the degree of malignancy of gliomas. rIL 6 can stimulate the proliferation of glioma cells, while IL 6 McAb can inhibit the proliferation of glioma cells. Conclusion Human glioma cells can also secrete and express IL 6 spontaneously. There is also an autocrine loop of IL 6 in human gliomas. The existence of this autocrine loop contributes to maintaining the malignant proliferation of gliomas.