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在无溶剂及二(2-乙基己基)丁二酸酯磺酸钠(AOT)/异辛烷/磷酸盐缓冲液微乳液体系中,研究了黑曲霉脂肪酶催化红花油水解反应的动力学.结果表明,无溶剂及微乳液体系中反应的活化能分别为32.205和7.391kJ/mol.酶在无溶剂体系中的热稳定性高于微乳液中.无溶剂及微乳液体系中的表观米氏常数分别为0.135和0.101mol/L.在两种体系中,乙醇对水解反应的抑制作用均为竞争性可逆抑制,且均在底物浓度大于0.819mol/L时出现底物抑制现象.结合胶团催化理论和酯键水解机理对两种体系中酶水解性能的差异进行了解释
In a solvent-free and AOT / isooctane / phosphate buffer microemulsion system, the kinetics of the Aspergillus niger lipase catalyzed hydrolysis of safflower oil was studied The results showed that the activation energies of the reactions in the solvent-free and microemulsion systems were 32.205 and 7.391 kJ / mol, respectively, and the thermal stability of the enzyme in the solvent-free system was higher than that in the microemulsion. View the Michaelis constant were 0.135 and 0.101mol / L. In both systems, the inhibitory effect of ethanol on the hydrolysis were competitive reversible inhibition, and substrate concentrations were greater than 0.819mol / L when the phenomenon of substrate inhibition The differences between the enzymatic hydrolysis properties of the two systems were explained by the theory of micellar catalysis and ester bond hydrolysis