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目的对2008～2010年青海省流感病毒监测结果进行分析,及时发现变异病毒和新型毒株,预测流感流行趋势。方法采用Real-time PCR方法进行流感病毒检测,采用MDCK细胞培养法分离流感病毒。结果 2008～2010年青海省收集监测标本9 882份,分离流感病毒2 347株,分离率23.75%;2008年分离64株流感病毒,其中H1N1亚型56株,H3N2亚型6株,B型2株;2009年检出新甲型H1N1阳性2 034份,分离流感毒株58株,其中H1N1亚型31株,H3N2亚型17株,B型10株;2010年检出病毒218株,其中季节性H1N1亚型4株,季节性H3N2亚型140株,B型61株,甲型H1N1 13株。结论 2008年季节性H1N1亚型为优势株,2009年优势株为甲型H1N1流感,2010年为H3N2亚型。
Objective To analyze the surveillance results of influenza virus in Qinghai Province from 2008 to 2010, and find out the mutant viruses and new strains in time and predict the epidemic trend of influenza. Methods Real-time PCR was used to detect influenza virus, and influenza virus was isolated by MDCK cell culture. Results A total of 9 882 surveillance samples were collected from 2008 to 2010 in Qinghai Province. 2 347 influenza viruses were isolated and the isolation rate was 23 75%. Sixty-four influenza viruses were isolated in 2008, including 56 H1N1 subtypes, 6 H3N2 subtypes, and 2 B-2 In 2009, 2 034 positive samples of new type A H1N1 were isolated and 58 strains of influenza were isolated, of which 31 were H1N1, 17 were H3N2 and 10 were type B; 218 strains were detected in 2010, of which the season Four H1N1 subtypes, 140 seasonal H3N2 subtypes, 61 B type and 13 H1N1 strains. Conclusion The seasonal H1N1 subtype was the predominant strain in 2008, the dominant strain was H1N1 influenza in 2009 and the H3N2 subtype in 2010.