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目的在参展青岛世界园艺博览会的荷兰进口马蹄莲植株上,发现叶片有明显褐色软腐症状,为明确引起马蹄莲软腐病的病原,对叶片进行病菌的分离鉴定。方法采用组织分离法,从荷兰入境马蹄莲植株的病变叶片中分离纯化到2株致病性细菌菌株(MTL1、MTL2),对该菌株进行鉴定和生物学特性研究。结果形态及培养特征、生理生化特性的研究结果表明该菌株在NA培养基上能形成白色圆形单菌落,薄而平滑、边缘整齐,光滑不透明,KB培养基上划线培养后可产生明显的绿色的荧光。显微镜下菌体呈杆状,具多根极生鞭毛,革兰氏阴性菌。结论经形态鉴定、培养特征、生理生化及16S r DNA序列分析和致病性测定实验,确认引起马蹄莲细菌性软腐病的病原为边缘假单胞菌(Pseudomonas marginalis)。
Objective To observe the obvious brown soft-rot symptoms on the plants of Holland imported into the Qingdao International Horticultural Exposition. To clearly identify the pathogen of the soft rot of calla lily, the pathogen was isolated and identified. Methods Tissue isolation method was used to isolate and purify two pathogenic bacterial strains (MTL1 and MTL2) from diseased leaves of the Netherlands calla lily plants. The identification and biological characteristics of the strains were studied. Results Morphological and cultural characteristics, physiological and biochemical characteristics of the strain showed that the strain could form a white circular single colony on NA medium, which was thin and smooth, neat edge, smooth and opaque. The KB culture medium could produce obvious Green fluorescent. Microscopic bacteria was rod-shaped, with multiple polar flagella, Gram-negative bacteria. Conclusion The pathogenicity of Bacterial soft rot caused by callus was identified as Pseudomonas marginalis by morphological identification, culture characteristics, physiological and biochemical analysis, 16S r DNA sequence analysis and pathogenicity test.