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目的 :了解喉乳头状瘤组织内 HPV1 6 / 1 8的感染与抑癌基因 p5 3变异的关系 ,以及 HPV感染在喉乳头状瘤发病中的作用。方法 :采用 PCR和免疫组化技术 ,检测 35例喉乳头状瘤组织中 HPV1 6 / 1 8DNA及 p5 3蛋白的表达。结果 :2 4例组织中检出 HPV1 6 / 1 8DNA(6 8.6 % ) ;19例 p5 3蛋白呈过度表达 (5 4.3% ) ;在 12例中同时检出HPV1 6 / 1 8DNA和 p5 3蛋白过度表达 (34 .3% )。结论 :提示 HPV1 6 / 1 8感染和 p5 3变异与喉乳头状瘤的发生明确相关 ,其内在分子机理及临床意义有待进一步阐明。
Objective: To investigate the relationship between HPV16 / 18 infection and the mutation of p65 gene in laryngeal papilloma and the role of HPV infection in the pathogenesis of laryngeal papilloma. Methods: The expression of HPV16 / 18 DNA and p53 protein in 35 cases of laryngeal papilloma was detected by PCR and immunohistochemistry. Results: The HPV16 / 18 DNA (26.6%) was detected in 24 cases and the expression of p5-3 protein in 19 cases (5.33%). The HPV16 / 18 DNA and p5-3 protein were detected in 12 cases Overexpression (34.3%). Conclusions: The results suggest that HPV16 / 18 infection and p5-3 mutation have a clear correlation with the occurrence of laryngeal papilloma. The intrinsic molecular mechanism and clinical significance need to be further elucidated.