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目的:探讨川麦冬和浙麦冬的生态型差异,为道地药材生物学实质的深入研究提供参考。方法:选择传统道地药材麦冬为模型,收集75份实验样本,通过分子鉴定技术和生态因子分析研究川麦冬和浙麦冬的生态型差异。结果:川麦冬和浙麦冬的psbA-trnH序列在49位点处存在变异,川麦冬为碱基腺嘌呤(A),浙麦冬为碱基鸟嘌呤(G),此为两者序列的差异化特征;生态因子分析显示四川、浙江两大产区呈现不同的地理生态型。其中,浙江产区在年均日照强度、年均降水量、年均相对湿度、最热季平均温度、生长期平均温度等因子上高于四川,而四川产区的最冷季平均温度高于浙江。结论:川麦冬和浙麦冬的基因型和生态因子均存在明显差异。
OBJECTIVE: To explore the differences of ecotypes between Chuanmai winter and Zhejiang Ophiopogon japonicus, and to provide reference for further study on the essence of Daoxing herbs. Methods: Radix Ophiopogon japonicus, a traditional Chinese medicinal herb, was selected as the model and 75 experimental samples were collected. The ecological differences of Chuanmai winter and Zhejiang Ophiopogon japonicus were studied by molecular identification techniques and ecological factors analysis. Results: The psbA-trnH sequence of Chuanmai winter and Zhejiang Ophiopogon japonicus showed variation at 49 locus, Chuanmai winter was basic adenine (A), and Zingiber officinale was basic guanine (G) The results of ecological factor analysis showed that the two producing areas of Sichuan and Zhejiang showed different geographical ecotypes. Among them, the producing areas in Zhejiang Province are higher than those of Sichuan in average annual sunshine intensity, average annual precipitation, annual average relative humidity, the hottest season average temperature and the average growing season temperature, while the coldest season temperatures in Sichuan are higher than Zhejiang. Conclusion: There are significant differences in genotypes and ecological factors between Chuanmai winter and Zhejiang Ophiopogon japonicus.