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为探讨胆囊收缩素八肽（ｃｈｏｌｅｃｙｓｔｏｋｉｎｉｎ ｏｃｔａｐｅｐｔｉｄｅ， ＣＣＫ－８）及中毒剂量谷氨酸（ｇｌｕｔａｍａｔｅ， Ｇｌｕ）对大鼠大脑皮质细胞内Ｃａ２＋浓度（［Ｃａ２＋］ｉ）的影响及相互关系，以１０－１０、 １０－８、 １０－６ｍｏｌ／Ｌ等浓度ＣＣＫ－８和１ｍｍｏｌ／Ｌ Ｇｌｕ分别或共同作用于大鼠大脑皮质驯胞，以流式细胞仪（ Ｆｌｏｗ ｃｙｔｏｍｅｔｅｒ， ＦＣＭ）检测［Ｃａ２＋］ｉ，并探讨其变化机制。① １０－１０ ｍｏｌ／ＬＣＣＫ－８可增加［Ｃａ２＋］ｉ（Ｐ＜０．０５）， １０－８、１０－６ｍｏｌ／ＬＣＣＫ－８无此作用；②１０－１０ｍｏｌ／ＬＣＣＫ－８与１ｍｍｏｌ／Ｌ Ｇｌｕ共同作用可增加［Ｃａ２＋］ｉ（Ｐ＜０．０５），而１０－６ｍｏ１／ＬＣＣＫ－８不引起［Ｃａ２＋］ｉ增加；③当抑制细胞内钙库释放时，１０－１０ｍｏｌ／ＬＣＣＫ－８加１ｍｍｏｌＬ Ｇｌｕ不能增加［Ｃａ２＋］ｉ。结果提示ＣＣＫ－８生理浓度时可增加［Ｃａ２＋］ｉ，而高浓度时无此作用；ＣＣＫ－８在增加［Ｃａ２＋］ｉ的同时有拮抗 Ｇｌｕ引起的细胞外钙内流作用。
To investigate the effect of cholecystokinin octapeptide (CCK-8) and glutamate (Glu) on the intracellular Ca2 + concentration ([Ca2 +] i) in rat cerebral cortex, The concentrations of CCK-8, 10 mmol / L, 10-6 mol·L-1 and 1 mmol / L Glu, respectively, were applied to rat cerebral cortex and detected by flow cytometer (FCM) And explore its mechanism of change. ①10-10mol / L CCK-8 could increase [Ca2 +] i (P <0.05), while10-8mol / L CCK-810-8mol / L (P <0.05), while 10-6mo1 / LCCK-8 did not cause [Ca2 +] i increase; ③ When inhibiting intracellular calcium release, 10-10mol / L CCK-8 plus 1 mmol L Glu does not increase [Ca 2+] i. The results suggested that [Ca2 +] i increased at the concentration of CCK-8, but no effect at high concentration. CCK-8 could antagonize Glu-induced extracellular calcium influx while increasing [Ca2 +] i.