论文部分内容阅读
目的 探讨树突状细胞 (DC)体外诱导的抗肿瘤免疫在肝癌患者体内的抗转移作用。方法 分离肝癌患者外周血 DC及 T淋巴细胞 ,Hep G2细胞抗原激活 DC,粒 /巨噬细胞集落刺激因子和白细胞介素 - 4联合刺激DC,DC诱导自体 T淋巴细胞增殖分化为细胞毒性 T细胞 (CTL) ,DC及其诱导的 CTL 回输肝癌患者 ,检测肝癌患者外周血 AFP m RNA。结果 AFP m RNA阳性组经 DC及其诱导的 CTL 治疗 2月后 ,治疗组 9例 AFP m RNA阳性患者中 7例转为阴性 ,对照组 9例 AFP m RNA仍全部为阳性 ;AFP m RNA阴性组经 DC及其诱导的 CTL 治疗2月后 ,治疗组 11例 AFP m RNA仍为阴性 ,而对照组 10例中 6例 AFP m RNA转为阳性。结论 DC诱导的抗肿瘤免疫能抑制肝癌转移。
Objective To investigate the anti-metastatic effects of dendritic cells (DCs) induced anti-tumor immunity in patients with hepatocellular carcinoma. Methods DC and T lymphocytes in peripheral blood of patients with hepatocellular carcinoma were isolated, Hep G2 cell antigen activated DC, Granulocyte/macrophage colony stimulating factor and Interleukin-4 were combined to stimulate DC, DC induced autologous T lymphocyte proliferation and differentiation into cytotoxic T cells. (CTL), DC and its induced CTL return liver cancer patients, detection of AFP m RNA in peripheral blood of patients with liver cancer. Results After AFP m RNA positive group was treated with DC and its induced CTL for 2 months, 7 cases of 9 cases of AFP m RNA positive in treatment group were negative, and 9 cases of AFP m RNA in control group were still all positive; AFP m RNA was negative. After 2 months of treatment with DC and its induced CTL, 11 cases of AFP m RNA were still negative in the treatment group, while 6 out of 10 cases in the control group became positive for AFP m RNA. Conclusion DC-induced anti-tumor immunity can inhibit the metastasis of liver cancer.