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根据大豆共生特异性,采取“菌株一品种”组合选择途径,从大豆品种猴子毛根瘤中分离和筛选出了大豆根瘤菌113—2。该菌为典型慢生(MGT=9.33小时)根瘤菌(Bradyrhi-zobium),不同于12个供试抗体/菌株的血清型。水培盆栽试验表明,在春(矮脚早)、夏或秋(猴子毛)播大豆上,比有效菌005、CB1809结瘤竞争力强;具有能量再利用的高效率固氮的氢吸收系统(Hup~+);在供试8个品种上均有效固氮,生长40天的地上植株干重平均为不接种的2倍,净固定氮为55.22mgN/株,比005(在矮脚早上)、B15和CB1809(在4个南方推广品种上)有效固氮(干重)更显著(P<0.05),比美国最活性菌株USDA110的自生吸H_2提高一倍(P<0.05)。田间菌比试验,在泰兴黑上比B15显著(P<0.05)增产50斤/亩,23％,在矮脚早上比005增产29.3斤/亩,13％。在春播矮脚早、泰兴黑和夏(秋)播鄂豆2号、猴子毛等4个南方推广品种上均比不接种显著(P<0.05)增产,每亩增收大豆24.7—55.0斤,增产8—26.2％。113—2单一菌剂接种春、夏、秋播大豆多点试验,比不接种分别增产13.7、10.8、18.5％(P<0.01)。大豆根瘤菌113—2已供应全国18个省、市、区、83个科研、教学、生产单位试验研究,据不完全统计,已应用面积7万亩以上。春、夏、秋播大豆共87个试验、示范结果,增产次数占90％以上,平均每?
According to the symbiosis specificity of soybean, soybean rhizobium strain 113-2 was isolated and screened from the soybean root nodule by adopting the combination of “strain and variety”. The bacterium is a typical slow-growing (MGT = 9.33 hours) Bradyrhi-zobium, different from the serotypes of 12 tested antibodies / strains. Pot experiments in hydroponic culture showed that they were more competitive than the effective bacteria 005 and CB1809 in sowing the spring (avalanches), summer or autumn (monkey fur) sowing, and high efficiency nitrogen fixing hydrogen absorption system with energy reuse Hup ~ +). The average dry weight of above-ground plants grown for 40 days was twice that of non-inoculated plants, and the net fixed nitrogen was 55.22mgN / plant, which was higher than that of 005 (short day) The effective nitrogen fixation (dry weight) of B15 and CB1809 (on four southern popularized cultivars) was more significant (P <0.05), which was twice as much as that of USDA110 (P <0.05). In the field experiment, the yield was increased by 50 kg / mu and 23% in Taixing black compared with that in B15 (P <0.05), yielding 29.3 kg / mu and 13% higher than that in 005. The yield was increased significantly in spring sowing bantam, Taixinghei and summer (autumn) sowing E 2, monkey hair and other four southern popularized varieties than non inoculation (P <0.05), increasing yield by 24.7-55.0 kg per acre and increasing yield 8-26.2%. 113-2 single inoculation of spring, summer and autumn seeding multi-point test, respectively, than the non-inoculation increased yield 13.7,10.8,18.5% (P <0.01). Soybean rhizobia 113-2 has been supplied to 83 research, teaching and production units in 18 provinces, cities and districts in China. According to incomplete statistics, the area has been applied more than 70,000 mu. Spring, summer and autumn sowing a total of 87 experiments, demonstration results, the number of production increases accounted for more than 90% of the average?