本文应用国产~(125)碘化钠标记的单碘胰岛素,改良经典的Gambhir方法,测定人红细胞胰岛素受体获得成功。标记胰岛素和受体的最大特异性结合率为15.42±4.0%,每个红细胞胰岛素受体的高结合力结合位点7～56,均值为21±15个,亲合常数K_1为(1.03±0.49)×10~9LM~(-1);低亲合力结合位点708-24705个,均值为8072±8055个,亲合常数K_2为(5.59±4.51)×10~5LM~(-1)。本实验采用计算机处理Scatchard曲线,避免了数据处理中的主观误差。 In this paper, Iodine-labeled monoiodo-insulin labeled with 125I sodium iodide was modified to improve the classical Gambhir method to determine the success of human erythrocyte insulin receptor. The maximum specific binding rate of labeled insulin to the receptor was 15.42 ± 4.0%. The binding affinity of each red blood cell insulin receptor was from 7 to 56 with an average of 21 ± 15 and the affinity constant K_1 was (1.03 ± 0.49 ) × 10 ~ 9LM -1. The number of low affinity binding sites was 708-24705, with an average of 8072 ± 8055. The affinity constant K_2 was (5.59 ± 4.51) × 10 ~ 5LM -1. In this experiment, computer processing Scatchard curve, to avoid subjective errors in data processing.