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目的探讨白细胞介素9(IL-9)在原发性胆汁性肝硬化(PBC)患者中的表达水平及其临床意义。方法选取80例PBC患者和100例健康人,采用流式微珠阵列(CBA)法检测血清IL-9、γ干扰素(IFN-γ)、IL-6、肿瘤坏死因子-α(TNF-α)、IL-10和IL-17水平,实时荧光定量PCR检测外周血单个核细胞(PBMC)IL-9 mRNA水平,免疫组织化学染色检测肝组织IL-9表达和定位。结果与健康人相比,PBC患者血清IL-9、TNF-α、IL-6、IL-17、IFN-γ、IL-10均显著升高,且IL-9与Ig G水平正相关。PBC患者外周血中IL-9 mRNA水平升高,PBC肝组织中有IL-9阳性细胞,主要位于汇管区的胆管上皮细胞。结论 PBC患者外周血和肝组织中高表达IL-9。
Objective To investigate the expression of interleukin-9 (IL-9) in patients with primary biliary cirrhosis (PBC) and its clinical significance. Methods Eighty PBC patients and 100 healthy volunteers were enrolled in this study. Serum levels of IL-9, IFN-γ, IL-6 and TNF-α were measured by flow cytometry (CBA) , IL-10 and IL-17 levels in peripheral blood mononuclear cells (PBMCs) were detected by real-time fluorescence quantitative PCR. The expression of IL-9 in liver tissues was detected by immunohistochemical staining. Results Serum levels of IL-9, TNF-α, IL-6, IL-17, IFN-γ and IL-10 in PBC patients were significantly higher than those in healthy controls. The levels of IL-9 mRNA in peripheral blood of patients with PBC increased, IL-9 positive cells in PBC liver tissue, mainly located in the portal area of bile duct epithelial cells. Conclusion The expression of IL-9 is high in peripheral blood and liver of PBC patients.