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目的：研究胃癌ｃｅｒｂＢ２ 基因扩增。方法：使用荧光原位杂交技术（ＦＩＳＨ） 检测５５ 例胃癌。结果：胃癌肿瘤区ｃｅｒｂＢ２基因扩增阳性率为３６－４％ 。在胃癌移行区内阳性率为１０－９％ ，在正常粘膜区阳性率为３－６ ％ ，相互比较，三个区域之间差异有高度显著性（ Ｐ＜ ０－０１）。在肠型和弥漫型胃癌，ｃｅｒｂＢ２ 基因扩增阳性率为５１－６％ 和１６－７％ ，两者差异有显著性（ Ｐ＜０－０５）。ｃｅｒｂＢ２ 基因扩增阳性与淋巴结内胃癌转移有明显相关性（ Ｐ＜ ０－０５）。结论：ｃｅｒｂＢ２ 基因扩增与胃癌的发生，发展有明显关联，有助于判断胃癌预后。
Objective: To study the cerbB2 gene amplification in gastric cancer. METHODS: Fifty-five cases of gastric cancer were detected using fluorescence in situ hybridization (FISH). RESULTS: The positive rate of cerbB2 gene amplification in gastric cancer tumor area was 36–4%. The positive rate in the transition zone of gastric cancer was 10-9%, and the positive rate in normal mucosa was 3-6 %. Compared with each other, the difference between the three regions was highly significant (P < 0-01). In intestinal and diffuse gastric cancer, the positive rate of c-erbB-2 gene amplification was 51-6% and 16-7%, and the difference was significant (P<0-05). Positive c-erbB 2 gene amplification was significantly correlated with gastric cancer metastasis in lymph nodes (P < 0-05). Conclusion: The amplification of cerbB2 gene is significantly associated with the occurrence and development of gastric cancer, which is helpful for judging the prognosis of gastric cancer.