论文部分内容阅读
免疫表型检测已成为白血病诊断和分类的重要手段。为了解急性髓细胞白血病(AML)免疫表型特征,利用 三色流式细胞术CD45／SSC参数散点图设门方法,对115例AML患者幼稚细胞表面及胞浆内分化抗原进行分析;结 合FAB分类,比较AML不同亚型中抗原表达的差异,并对其诊断价值加以探讨。结果显示:在AML患者中,CD33、 CD38和CD13的表达最常见,分别达94.8％、91.3％、89.6％。在淋系抗原中,CD7的表达最为常见(20.2％),其次是 CD19(16.5%)和CD2(15％)。某些免疫表型特征与FAB分类具有相关性,包括M3中缺乏表达HLA-DR、CD34和 CD56,但CD2的表达增加;M2中CD19,M5中CD14和CD56的表达增加,而M0中未见MPO的表达。结论:多参数 流式细胞术是诊断AML的一项可靠技术,AML某些免疫表型特征与FAB分类具有明显相关性。
Immunophenotyping has become an important tool for the diagnosis and classification of leukemia. In order to understand the immunophenotypic characteristics of acute myeloid leukemia (AML), we analyzed the immature cell surface and intracytoplasmic differentiation antigen in 115 cases of AML by using the three-color flow cytometry CD45 / SSC scattergram method. FAB classification, comparison of different subtypes of AML antigen expression differences, and its diagnostic value to be explored. The results showed that the expression of CD33, CD38 and CD13 were the most common in AML patients, accounting for 94.8%, 91.3% and 89.6% respectively. Of the lymphoid antigens, CD7 expression was the most common (20.2%), followed by CD19 (16.5%) and CD2 (15%). Some immunophenotypic features were associated with FAB classification, including the lack of expression of HLA-DR, CD34 and CD56 in M3, but increased expression of CD2; the expression of CD14 and CD56 in CD19 and M5 was increased in M2 but not in M0 MPO expression. Conclusion: Multiparameter flow cytometry is a reliable technique for diagnosing AML. Some of the immunophenotypic characteristics of AML are significantly correlated with FAB classification.