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目的:研究九里香叶的药效物质基础。方法:利用制备薄层、反复硅胶色谱、半制备HPLC等方法进行分离纯化,根据理化性质及波谱数据对分离的化合物进行结构鉴定。结果:分离得到11个化合物,分别鉴定为5,7,3’,4’-四甲氧基黄酮(1),5,7-二羟基-3’,4’,5’-三甲氧基黄酮(2),5-羟基-3,7,3’,4’-四甲氧基黄酮(3),3-羟基-5,6,7,4’-四甲氧基黄酮(4),川陈皮素(5),香草酸(6),咖啡酸乙酯(7),5-羟基-7,3’,4’-三甲氧基黄酮(8),3,5-二羟基-6,7,3’,4’-四甲氧基黄酮(9),5,6,4-三羟基-3,7,-二甲氧基黄酮(10),山柰酚(11)。结论:3,4,6,9,10为首次从九里香叶中分离得到。
Objective: To study the pharmacological substance foundation of Grifola frondosa. Methods: The compounds were isolated and purified by preparative TLC, repeated silica gel chromatography and semi-preparative HPLC. The structures of the isolated compounds were identified by physicochemical properties and spectral data. Results: Eleven compounds were isolated and identified as 5,7,3 ’, 4’-tetramethoxyflavone (1), 5,7-dihydroxy-3’, 4 ’, 5’-trimethoxyflavone (2), 5-hydroxy-3,7,3 ’, 4’-tetramethoxyflavone (3), 3-hydroxy-5,6,7,4’-tetramethoxyflavone (4) (5), vanillic acid (6), ethyl caffeate (7), 5-hydroxy-7,3 ’, 4’-trimethoxyflavone (8), 3,5-dihydroxy-6,7 , 3 ’, 4’-tetramethoxyflavone (9), 5,6,4-trihydroxy-3,7-dimethoxyflavone (10) and kaempferol (11). Conclusion: 3, 4, 6, 9 and 10 were isolated from Grifola frondosa for the first time.