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目的研究醒脑静注射液对脓毒症大鼠心肌酶和线粒体呼吸链复合物的影响。方法按照体重将SD大鼠随机分为3组:正常组10只,腹腔注射与脂多糖(LPS)等量的0.9%NaCl;其余SD大鼠腹腔注射10 mg·kg~(-1)脂多糖(LPS),建立脓毒症模型。模型组与实验组均30只,实验组经胃管注入醒脑静注射液6.4 mL·kg~(-1)。于给药后6,24,48 h,用试剂盒法检测不同时点各组的心肌酶和呼吸链复合物的活性,包括NADH-辅酶Q还原酶(complexⅠ)、琥珀酸-辅酶Q还原酶(complexⅡ)、辅酶Q-细胞色素C还原酶(complexⅢ)、细胞色素C-氧化还原酶(complexⅣ)。结果在24 h,实验组与模型组的血清肌酸激酶分别为(1333.20±140.87),(1813.50±243.72)U·L~(-1);这2组的肌酸激酶同工酶分别为(748.15±119.02),(1167.92±145.17)U·L~(-1)。实验组的肌酸激酶、肌酸激酶同工酶水平均显著低于模型组,差异均有统计学意义(P<0.01)。在24 h,实验组和模型组的心肌线粒体complexⅠ活性分别为(2.83±0.15),(1.84±0.18)u·mg~(-1),这2组的complexⅢ活性分别为(0.91±0.13),(0.68±0.16)u·mg~(-1),这2组的complexⅣ分别为(19.43±1.37),(15.20±1.79)u·mg~(-1),实验组心肌线粒体complexⅠ、Ⅲ、Ⅳ活性均显著高于模型组差异均有统计学意义(P<0.05)。结论醒脑静注射液可显著降低脓毒症大鼠心肌酶水平,提高脓毒症大鼠心肌线粒体complexⅠ、Ⅲ及Ⅳ活性。
Objective To investigate the effects of xingnaojing injection on myocardial enzyme and mitochondrial respiratory chain complex in septic rats. Methods SD rats were randomly divided into 3 groups according to body weight: 10 normal rats were injected intraperitoneally with 0.9% NaCl equal to lipopolysaccharide (LPS); the other SD rats were injected intraperitoneally with 10 mg · kg -1 lipopolysaccharide (LPS), the establishment of sepsis model. Thirty rats in model group and experiment group were injected with 6.4 mL · kg ~ (-1) of Xingnaojing injection via gastric tube in experimental group. At 6, 24 and 48 h after administration, the activities of myocardial enzymes and respiratory chain complexes at different time points were detected by kit method, including NADH-coenzyme Q reductase (complexⅠ), succinate-coenzyme Q reductase (complex II), coenzyme Q-cytochrome C reductase (complex III), cytochrome C-oxidoreductase (complex IV). Results Serum creatine kinase in the experimental and model groups was (1333.20 ± 140.87) and (1813.50 ± 243.72) U · L -1 at 24 h, respectively. The creatine kinase isoenzymes in these two groups were ( 748.15 ± 119.02), (1167.92 ± 145.17) U · L -1. The creatine kinase and creatine kinase isoenzyme levels in the experimental group were significantly lower than those in the model group, with statistical significance (P <0.01). At 24 h, the activities of complex I in the mitochondria of experimental group and model group were (2.83 ± 0.15) and (1.84 ± 0.18) u · mg -1, respectively. The activity of complex Ⅲ in these two groups were (0.91 ± 0.13) and (0.68 ± 0.16) u · mg ~ (-1). The complex Ⅳ of the two groups were (19.43 ± 1.37) and (15.20 ± 1.79) u · mg ~ (-1) Activity were significantly higher than the model group differences were statistically significant (P <0.05). Conclusion Xingnaojing Injection can significantly reduce myocardial enzyme levels in septic rats and increase the activity of mitochondrial complex Ⅰ, Ⅲ and Ⅳ in septic rats.