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目的:观察黄芪对缺血性急性肾功能衰竭(ARF)时血清肝细胞生长因子(HGF)水平、肾功能及肾组织病理学改变的影响,探讨黄芪肾脏保护作用的机制。方法:通过切除大鼠右肾,夹闭左肾动脉60 min后再灌注(I/R),建立IARF模型。治疗组术前3 d连续给予黄芪ip,其他组(假手术组及I/R 0、4、12 h组)应用生理盐水。测量各组血清HGF、尿素氮(BUN)、肌酐(Cr)水平,并通过光镜下肾小管评分来评价肾组织病理学改变。结果:I/R 4 h对照组血清HGF水平与假手术组无显著差异(P>0.05);黄芪组血清HGF水平较I/R 4 h对照组明显升高(P<0.01);I/R对照组血清BUN、Cr水平较假手术组明显升高(P<0.01),黄芪组可以减少血清BUN、Cr水平升高的程度(P<0.05);同时也可以使肾组织病理损伤显著改善(P<0.05)。结论:在IARF模型,黄芪预处理可以减轻肾脏损伤,改善肾功能。黄芪诱导HGF生成增加可能是其肾脏保护作用的机制之一。
Objective: To observe the effects of Astragalus membranaceus on serum hepatocyte growth factor (HGF) level, renal function, and renal histopathological changes during ischemic acute renal failure (ARF), and to explore the mechanism of renal protective effects of Astragalus membranaceus. METHODS: The IARF model was established by removing the right kidney of rats and clamping the left renal artery for 60 minutes followed by reperfusion (I/R). The treatment group received ip administration of jaundice 3 days before surgery, and normal saline was used in other groups (sham operation group and I/R 0, 4 and 12 h groups). The levels of serum HGF, urea nitrogen (BUN) and creatinine (Cr) were measured in each group, and renal pathological changes were evaluated by light microscopy renal tubules score. Results: There was no significant difference in serum HGF levels between the I/R 4 h control group and the sham group (P>0.05). Serum HGF levels in the Astragalus membranaceus group were significantly higher than those in the I/R 4 h control group (P<0.01); I/R Serum BUN and Cr levels in the control group were significantly higher than those in the sham operation group (P<0.01). Huangqi group could reduce the increase of serum BUN and Cr levels (P<0.05); at the same time, it could also significantly improve renal pathological injury ( P<0.05). Conclusion: In the IARF model, Astragalus pretreatment can reduce kidney damage and improve renal function. Astragalus induces increased production of HGF may be one of the mechanisms of its renal protective effect.