此方法是在对PCR扩增DNA进行手工直接因相排序后.所得排序产物可用无放射性的方法检测.主要步骤和原理:首先,对ber-abl接位点进行PCR扩增.用于扩增的低聚核甙酸有两个引物,在其中一个引物的5’末端,已经扩增待研究的DNA片段被生物素化,并可与链球菌抗生素蛋白包裹的磁珠配对.而固定双股DNA通过碱处理后,分裂成单股DNA.一 This method is based on the manual PCR directly after the phase sequence of DNA sequencing products obtained can be non-radioactive detection methods and procedures: First, the ber-abl access sites for PCR amplification for amplification Of the oligonucleotides have two primers at the 5 ’end of one of the primers, the DNA fragment that has been amplified to be studied is biotinylated and can be paired with the beads coated with the streptococcal antibiotic protein, while the immobilized double stranded After the DNA is treated with alkali, it splits into single-stranded DNA