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本文用CCl_41.356g/kg和5.87g/kg分别对大鼠进行皮下和呼吸道静式染毒,为期8周亚急性中毒试验,研究临床常用血清肝酶指标的变化。结果发现:CCl_4除使大鼠体重增长减慢外,第1周起出现肝细胞脂变、浊肿,进而坏死、纤维增生和肝硬化;肝糖元及SHD酶活性减少或消失,G-6-P酶活性先升高后降低的病理形态和组织化学的改变。与此同时或稍后出现SGPT和SGOT活性升高,持续至第8周。停药两周,肝病理改变趋于恢复,SGPT和SGOT活性也恢复至接近正常,两肝酶与病理改变相平行。AKP酶活性第4周后才升高;ChE酶似有先升高后降低趋势,但无明显差异性;γ-GT酶变化不规则。提示CCl_4亚急性中毒时,SGPT和SGOT酶活性升高与肝损关系较密切,可作临床早期诊断指标。血清AKP和ChE酶亦一定程度反映肝损的发展情况,可供作临床观察病情发展的辅助指标。
In this paper, CCl_41.356g / kg and 5.87g / kg rats were subcutaneously and respiratory tract static exposure, a 8-week subacute poisoning test to study the changes of clinical serum liver enzymes. The results showed that, in addition to slowing the body weight gain of CCl_4 rats, the first week of hepatocyte lipidosis, turbidity, and then necrosis, fibrosis and cirrhosis; liver glycogen and SHD enzyme activity decreased or disappeared, G-6 -P enzyme activity increased first and then decreased the pathological morphology and histochemical changes. At the same time or later SGPT and SGOT activity increased until the 8th week. After stopping for two weeks, the pathological changes of liver tended to recover, and the activity of SGPT and SGOT returned to normal. The two liver enzymes paralleled the pathological changes. AKP enzyme activity increased after 4 weeks; ChE enzyme first increased and then decreased, but no significant difference; γ-GT enzyme changes irregular. Prompted CCl 4 subacute poisoning, SGPT and SGOT enzyme activity and liver damage is closely related to the early diagnosis of indicators. Serum AKP and ChE enzymes also reflect the development of liver damage to some extent, which can be used as an auxiliary index for the clinical observation of disease development.