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目的比较葡萄汁与单体白黎芦醇的促排镉作用及其机制。方法小鼠饲喂CdCl2、白黎芦醇或不同浓度葡萄汁,4周后处死,测定肝、肾镉含量,测血清尿素氮(BUN)及谷丙转氨酶(ALT)的含量,测血清、肝脏、肾脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)的含量、谷胱甘肽过氧化物酶(GSH-Px)的活力。结果与染镉组相比,白黎芦醇、葡萄汁可促进肝、肾镉的排出,降低BUN、ALT的含量,降低血清、肝脏、肾脏MDA的含量,使SOD、GSH-PX的活力恢复,以上各指标中,葡萄汁随着浓度增大作用加强,低剂量组的作用比白黎芦醇组差,中、高剂量组的作用比白黎芦醇组强。结论葡萄汁对镉中毒小鼠肝、肾损伤的保护作用强于白黎芦醇,这可能与白黎芦醇和果汁中的其它植物化学物质发挥协同作用有关。
Objective To compare the mechanism of promoting and removing cadmium of grape juice and monomer resveratrol. Methods The mice were fed with CdCl2, resveratrol or grape juice at different concentrations. After 4 weeks, the mice were sacrificed and the contents of liver and kidney cadmium were measured. The levels of serum urea nitrogen (BUN) and alanine aminotransferase (ALT) , Renal superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity. Results Compared with cadmium exposure group, resveratrol and grape juice could promote the excretion of liver and kidney cadmium, reduce the content of BUN and ALT, decrease the content of MDA in serum, liver and kidney, and restore the activity of SOD and GSH-PX In the above indexes, grape juice increased with increasing concentration, the effect of low dose group was worse than that of resveratrol group, and the effect of medium and high dose group was stronger than that of resveratrol group. Conclusions Grape juice can protect liver and kidney from cadmium poisoning more than resveratrol, which may be related to the synergistic effect of resveratrol and other phytochemicals in fruit juice.