本文筛选出聚氯乙烯平片作载片固定细菌,再与一抗及 HRP 标记二抗温育,以化学发光免疫法测定了食品中沙门氏菌。以 P/N≥3作为阳性标准,测定了62份食品样品,结果与常规培养法相比,符合率为85.5%。如均不考虑增菌时间,用本法测定沙门氏菌仅需8～4h,约为传统方法所需时间的1/10。 This paper screened polyvinyl chloride flat films for cell immobilization bacteria, and then with the first antibody and HRP labeled secondary antibody incubation, chemiluminescence immunoassay for the determination of Salmonella in food. With P / N≥3 as the positive standard, 62 food samples were determined, and the result was 85.5% compared with the conventional culture method. If you do not consider the time of enrichment, using this method to measure Salmonella only 8 ~ 4h, about the time required for the traditional method of 1 /