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目的探讨钉螺人工群体感染血吸虫的优化时间。方法在实验室条件下,采用日本血吸虫毛蚴分别感染钉螺2、3、4 h 3组,观察感染60~120 d后各组钉螺死亡率、钉螺感染率及尾蚴获得量,分析各组3个指标的差异,以钉螺死亡率相对低、钉螺感染率和尾蚴获得量相对高的钉螺感染时间为最优时间。结果3 h感染组60 d的钉螺感染率显著高于其他组(P<0.05),钉螺死亡率低于其他组(P<0.05),60~120 d的尾蚴获得量显著高于2 h感染组(P<0.05),与4 h感染组差异无统计学意义(P>0.05)。结论实验室条件下,血吸虫毛蚴人工群体感染钉螺3 h为相对优化的感染时间。
Objective To investigate the optimal time of schistosomiasis infection in the artificial population of Oncomelania hupensis. Methods In laboratory conditions, S. japonicum miracidia were infected with 3, 4 and 4 hours respectively to observe the mortality, the infection rate of snail and the amount of cercariae obtained in each group from 60 to 120 days after infection. Three indicators of each group , The relative mortality of Oncomelania snails was relatively low, and the infection time of snails and the relatively high amount of cercariae obtained from snails were the optimal time for infection. Results The infection rates of Oncomelania snails at 60 d after 3 h infection were significantly higher than those at other days (P <0.05), and the mortality of Oncomelania snails was lower than other groups (P <0.05) (P <0.05), but no significant difference with 4 h infection (P> 0.05). Conclusion Under laboratory conditions, infection of Schistosoma japonicum by artificial population of Schistosoma japonicum for 3 h is a relatively optimized infection time.