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应用免疫组化方法研究了p53蛋白在肺癌组织和胸水细胞中的表达情况。发现p53蛋白在常规石蜡切片和胸水中保存较好,在肺癌组织中表达的总阳性率为66.3%,正常细胞中未见p53蛋白表达;肺癌有淋巴结转移组p53蛋白的阳性率(75.5%)高于无淋巴结转移组(55.8%)(P＜0.05);Ⅲ—Ⅳ期肺癌p53蛋白的阳性率(82.9%)高于Ⅰ—Ⅱ期肺癌(56.1%)(P＜0.01)。结果表明,p53基因突变与肺癌的发生、淋巴结转移及进展有关,突变型p53蛋白是肺癌较特异的肿瘤标记物。
Immunohistochemistry was used to study the expression of p53 protein in lung cancer and pleural effusion. It was found that p53 protein was well preserved in routine paraffin sections and pleural effusions. The total positive rate of expression in lung cancer tissues was 66.3%. There was no p53 protein expression in normal cells. The positive rate of p53 protein in lung cancer with lymph node metastasis was 75.5%. The positive rate of p53 protein in stage III-IV lung cancer was higher than that in stage I-II lung cancer (56.1%) (P<0.01). The results showed that p53 gene mutations are associated with the occurrence of lung cancer, lymph node metastasis and progression. Mutant p53 protein is a specific tumor marker for lung cancer.