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目的:探讨运动训练对心理应激大鼠下丘脑-垂体-性腺(HPG)轴的影响。方法:将50只SD大鼠随机分为对照组、运动组、急性心理应激组、慢性心理应激组、急性心理应激+运动组、慢性心理应激+运动组。运动大鼠进行10周游泳运动(1次/天,60min/次);心理应激大鼠分别施加急(一次)、慢(后3周)性心理应激。测定各组大鼠下丘脑GnRHmRNA、垂体LHmRNA表达及血清睾酮(T)、皮质醇(C)含量。结果:(1)急、慢性心理应激组大鼠下丘脑GnRH、垂体LHmRNA表达量、血清T水平均显著低于对照组,血清C水平显著高于对照组;慢性心理应激组大鼠体重、睾丸重、睾丸重/体重均显著低于对照组。(2)与对照组相比,运动组大鼠下丘脑GnRH、垂体LH表达及血清T、C均无明显变化。(3)与心理应激组比,慢性心理应激+运动组大鼠下丘脑GnRH、垂体LH表达量以及血清T水平显著升高。结论:心理应激作为一种应激源,可能会引起大鼠HPG轴紊乱;中等负荷运动对提高机体抗应激能力有一定作用。
Objective: To investigate the effect of exercise training on the hypothalamus-pituitary-gonadal (HPG) axis in psychological stress rats. Methods: 50 SD rats were randomly divided into control group, exercise group, acute psychological stress group, chronic psychological stress group, acute psychological stress + exercise group, chronic psychological stress + exercise group. Exercise rats for 10 weeks of swimming (1 / day, 60min / times); psychological stress rats were impatient (once), slow (after 3 weeks) sexual psychological stress. The hypothalamus GnRHmRNA, pituitary LHmRNA, serum testosterone (T) and cortisol (C) were determined in each group. Results: (1) The levels of GnRH, pituitary LH mRNA and T level in hypothalamus of rats in acute and chronic psychological stress group were significantly lower than those in control group, serum C level was significantly higher than that in control group. The body weight of rats in chronic psychological stress group , Testicular weight, testicular weight / weight were significantly lower than the control group. (2) Compared with the control group, the expression of GnRH, pituitary LH, serum T and C in the hypothalamus of exercise group had no significant change. (3) Compared with psychological stress group, the expression of GnRH, pituitary LH and serum T level in chronic psychological stress + exercise group were significantly increased. Conclusion: Psychological stress as a stressor may cause disorder of HPG axis in rats. Moderate-load exercise may play an important role in improving the anti-stress ability of the body.