【摘 要】
：
Circular RNAs (circRNAs) are a new and large group of non-coding RNA molecules that are abundantly expressed in the central nervoussystem. However, very little is known about their roles in traumatic brain injury. In this study, we firstly screened differ
【机 构】
：
Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University,Chongqing,C
论文部分内容阅读
Circular RNAs (circRNAs) are a new and large group of non-coding RNA molecules that are abundantly expressed in the central nervoussystem. However, very little is known about their roles in traumatic brain injury. In this study, we firstly screened differentially expressed circRNAs in normal and injured brain tissues of mice after traumatic brain injury. We found that the expression of circLphn3 was substantially decreased in mouse models of traumatic brain injury and in hemin-treated bEnd.3 (mouse brain cell line) cells. After overexpressing circLphn3 in bEnd.3 cells, the expression of the tight junction proteins, ZO-1, ZO-2, and occludin, was upregulated, and the expression of miR-185-5p was decreased. In bEnd.3 cells transfected with miR-185-5p mimics, the expression of ZO-1 was decreased. Dual-luciferase reporter assays showed that circLphn3 bound to miR-185-5p, and that miR-185-5p bound to ZO-1. Additionally, circLphn3 overexpression attenuated the hemin-induced high permeability of the in vitro bEnd.3 cell model of the blood-brain barrier, while miR-185-5p transfection increased the permeability. These findings suggest that circLphn3, as a molecular sponge of miR-185-5p, regulates tight junction proteins\' expression after traumatic brain injury, and it thereby improves the permeability of the blood-brain barrier. This study was approved by the Animal Care and Use Committee of Chongqing Medical University of China (approval No. 2021-177) on March 22, 2021.
其他文献
Oscillating field stimulation(OFS)is a potential method for treating spinal cord injury.Although it has been used in spinal cord injury(SCI)therapy in basic and clinical studies,its underlying mechanism and the correlation between its duration and nerve i
Gene therapy aims to introduce genetic information into a cell-type of interest to replace, correct, silence, or modify defective genes. Gene therapy in its broadest sense can theoretically prevent, halt, or cure any condition that affects mankind. In add
What lurks within the crypt? With more than 200 known causes of ischemic stroke (Saver, 2016), what should be the minimum workup? Many centers offer high-resolution brain imaging and vessel studies, telemetry monitoring for atrial fibrillation (AF), trans
Developmental and epileptic encephalopathy 9 (DEE9): The gene PCDH19 (Xq22.1), which encodes the calcium-dependent cell adhesion protein protocadherin-19 (PCDH19), is nowadays considered as one of the most important genes in monogenic epilepsy (Depienne a
Zebrafish are an effective vertebrate model to study the mechanisms underlying recovery after spinal cord injury.The subacute phase after spinal cord injury is critical to the recovery of neurological function,which involves tissue bridging and axon regen
Arteriovenous malformation (AVM) is a vascular congenital defect affecting microvasculature of both brain and peripheral organs. Arteriovenous malformation of the brain (bAVM, OMIM #108010), in particular, affects up to 15 per 100,000 persons with no sex
Down syndrome (DS) is the most frequent chromosomal abnormality that causes intellectual disability, resulting from the presence of an extra complete or segment of chromosome 21 (HSA21) (Tramutola et al., 2020; Lanzillotta et al., 2021). Every year, appro
Lithium is associated with oxidative stress and apoptosis,but the mechanism by which lithium protects against spinal cord injury remains poorly understood.In this study,we found that intraperitoneal administration of lithium chloride(LiCl)in a rat model o
Alzheimer\'s disease (AD) is a progressive and irreversible neurodegenerative disorder that causes a decline of cognitive functions and a deterioration of behavioral and social performances. According to current estimations, AD is considered the prevale
The complex and mostly multiple and unknown aetiology of neurodegenerative diseases always give way to an intricate scenario of dying tissue that involves multiple cell mediators and cell types. All neurodegenerative diseases of the central nervous system