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病毒性肝炎是我国最重要的传染病之一,可导致严重慢性肝病,并且与肝细胞癌密切相关。近5年来,我们改进肝炎病毒核酸诊断技术,建立热变性HCVRNA直接扩增技术、RTPCR夹心斑点杂交技术、HBV前C区点突变检测技术,以及克隆HCV、HGV基因序列。此外,对广东甲型肝炎流行病学调查与输血传播HCV、HGV及TTV人群感染的情况、慢性HBV感染者病情反复发作的因素、HCV与肝癌关系、干扰素、Lamivudine的临床治疗观察、基因治疗的应用基础研究,以及中西医结合治疗病毒性肝炎等作了一系列研究。这些技术的改进与研究成果,迅速应用到临床防治工作中,对当前病毒性肝炎诊治工作起到积极的推动作用。
Viral hepatitis is one of the most important infectious diseases in our country. It can cause severe chronic liver disease and is closely related to hepatocellular carcinoma. In the past 5 years, we have improved the technology of hepatitis virus nucleic acid diagnosis, established direct thermal denaturation HCVRNA amplification technology, RT-PCR sandwich dot blot technology, point mutation detection of HBV pre-C region, and cloned HCV and HGV gene sequences. In addition, hepatitis C epidemiology in Guangdong and blood transfusion transmitted HCV, HGV and TTV crowd infection, chronic HBV infection recurrent disease factors, HCV and liver cancer, interferon, Lamivudine clinical observation, gene therapy Application of basic research, as well as Integrative Medicine for the treatment of viral hepatitis made a series of studies. The improvement of these technologies and research results, the rapid application of clinical prevention and treatment work, the current diagnosis and treatment of viral hepatitis play a positive role in promoting.