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提出一种利用回形针来制作三维微流控纸芯片的方法,能够适应多种不同的微流控纸芯片;利用自制的纸芯片比色检测装置,已用于牛血清白蛋白,Fe~(2+)的快速定量检测。牛血清白蛋白的线性范围是5~50μmol/L,检出限为0.13μmol/L,R~2=0.994。Fe~(2+)的线性范围是0.6~12μmol/L,检出限为0.15μmol/L,R~2=0.992。在所规定的范围内,都获得了较好的线性关系,验证了基于回形针的3D微流控纸芯片可用于实际样品检测的可能性。
A method of making three-dimensional microfluidic chip with paperclip is proposed, which can adapt to a great variety of different microfluidic chip. Using self-made paper chip colorimetric detection device, it has been used in bovine serum albumin, +) Rapid quantitative detection. The linear range of bovine serum albumin was 5 ~ 50μmol / L with the detection limit of 0.13μmol / L and R ~ 2 = 0.994. The linear range of Fe ~ (2+) was 0.6 ~ 12μmol / L with the detection limit of 0.15μmol / L and R ~ 2 = 0.992. In the specified range, have obtained a good linear relationship, validated the paperclip-based 3D microfluidic chip can be used for actual sample testing possibilities.