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目的运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用。方法以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用,采用流式细胞仪观察BS对谷氨酸所诱导PC12细胞凋亡的影响。结果BS含药血清可以增强PC12细胞活力,拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡。结论补肾方BS可能具有神经保护作用。
Objective To investigate the effects of kidney-enriched drug-containing serum (BS) on the proliferation of PC12 cells cultured in vitro and its effect on antagonizing glutamate-induced neurotoxicity injury using serum pharmacology of Chinese medicine. Methods MTT assay was used to observe the effect of BS on the viability of PC12 cells cultured in vitro and its effect on antagonizing glutamate injury. Flow cytometry was used to observe the effect of BS on the apoptosis of PC12 cells induced by glutamate. Results BS-containing serum could enhance the viability of PC12 cells, antagonize the excitatory neurotoxicity of glutamate and had the strongest effect at 20% concentration, and could inhibit the early apoptosis of PC12 cells induced by glutamate. Conclusion Bushenfang BS may have neuroprotective effect.