对农林院校《种子检验学》实验教学中关于种子质量检验实验所涉及系列问题,包括植物材料、分子标记、DNA分离、PCR条件、凝胶制备与电泳等方面问题进行了研究与探讨,提出了具体可行的实验方案,使分子标记技术适用于实验课堂教学操作应用,供从事相关实验的教师参考。 A series of questions about seed quality testing experiment, including plant materials, molecular markers, DNA isolation, PCR conditions, gel preparation and electrophoresis, were discussed and discussed in the experiment teaching of “seed testing” in agriculture and forestry colleges and universities The concrete feasible experiment scheme, makes the molecular marker technology suitable for the experimental classroom teaching operation application, the teacher reference which carries on the related experiment.