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红树林内生细菌及拮抗菌分离筛选结果表明:各品种红树体内均有大量的内生细菌,不同红树品种及部位内生细菌的数量不同,其中以红海榄体内的含量最高,达4.225×104cfu/g(fw),其它依次为木榄、桐花树、秋茄和白骨壤等;不同部位以茎组织体内内生细菌的含量最多,达1.649×104cfu/g(fw),其次为根和叶。获得的内生细菌中约有43.53%的内生细菌菌株对枯萎病菌(Fusarium oxysporum)、炭疽病菌(Colletotrichum sp.)等病原真菌及番茄青枯病菌(Ralstonia solanaceance)具有较强拮抗作用。对番茄生长测定发现,13株菌株中有9株(69.23%)表现为促进生长效果,4株(30.77%)表现为抑制生长作用。经初步鉴定,上述拮抗细菌均为芽孢杆菌(Bacillus sp.)。
Mangrove endophytic bacteria and antagonistic bacteria separation and screening results showed that: all varieties of mangrove species have a large number of endophytic bacteria, mangrove species and endophytic bacteria in different numbers, of which the highest content of Rhizophora, up to 4 , Followed by M. tuber, Aegiceras corniculatum, Kandelia canadensis and Avicennia marina were the most abundant in different parts of stem tissues, reaching 1.649 × 104 cfu / g (fw) , Followed by roots and leaves. About 43.53% of the endophytic bacteria in endophytic bacteria were antagonistic against pathogenic fungi such as Fusarium oxysporum, Colletotrichum sp. And Ralstonia solanaceance. The results of tomato growth assay showed that 9 out of 13 strains (69.23%) showed growth promoting effect and 4 (30.77%) showed growth inhibitory effect. Preliminary identification, the antagonistic bacteria are Bacillus (Bacillus sp.).