间接血凝试验已广泛应用于血清学试验。我们在探讨血凝用于血吸虫病诊断时,对其实验方法和影响因素进行了试验,现将结果报告如下。材料和方法一、抗原制备人工感染2,000条日本血吸虫尾蚴的家兔,42～45天解剖,取出肝脏,分离虫卵,冰冻干燥保存。使用时,将干卵研成粉末,用1/10,000硫柳汞生理盐水配成1%(W/V)浓度,在4℃中冷浸3～7天。用3,000rpm离心30分钟,上清液即为1:100虫卵抗原原液。用Lowry氏法测定蛋白含量为2mg/ml。保存于冰箱,备用。二、致敏红细胞的制备 (一)红细胞醛化取绵羊静脉血若干,注于装有玻璃珠的无菌瓶中,充分振摇,除去纤维蛋白;並在取血后当天按下列方法之一进行醛化。 1.甲醛化:取上述血细胞液,用尼龙绢 Indirect hemagglutination tests have been widely used in serological tests. When we discussed the use of blood coagulation in the diagnosis of schistosomiasis, we tested the experimental methods and influencing factors. The results are reported below. Materials and methods 1. Preparation of antigens Artificially infect 2,000 rabbits of the Japanese schistosome cercariae and dissect for 42 to 45 days. Remove the liver, isolate the eggs, freeze-dry and store. When used, the dry egg was ground into a powder and formulated with 1/10,000 thimerosal physiological saline to a concentration of 1% (w/v) and cold immersed for 3 to 7 days at 4°C. After centrifugation at 3,000 rpm for 30 minutes, the supernatant was 1:100 egg antigen stock. The protein content was determined to be 2 mg/ml using the Lowry’s method. Store in refrigerator, spare. Second, the preparation of sensitized red blood cells (a) red blood cell aldehyde to take a number of sheep venous blood, injection in a sterile glass vial, fully shaken to remove fibrin; and on the day after the blood according to one of the following methods Aldehyde. 1. Formaldehyde: Take the above blood cell fluid with nylon