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目的通过制备5/6肾切除(5/6 Nx)小鼠慢性肾脏病(CKD)模型,观察小鼠成纤维生长因子23(FGF23)及钙磷代谢指标,有助于研究矿物质与骨代谢紊乱的发病机制。方法 28只C57雄性小鼠适应性饲养1周后,切除模型组约5/6左肾,1周后切除右肾,16周后处死后收集血尿和肾脏组织标本,常规检测血尿FGF23、甲状旁腺素(PTH)、活性维生素D、钙、磷等指标,观察肾脏病理。结果与假手术组和正常对照组相比,术后16周5/6 Nx小鼠出现体质量减低,血清FGF23、PTH,血磷等异常升高,活性维生素D水平显著降低,尿蛋白增加。肾脏病理发现系膜基质增生、胶原沉积及纤维化明显,和肾小球代偿性肥大。结论 5/6 Nx小鼠可以出现明显的矿物质与骨代谢紊乱特征,是研究CKD骨病合适的模型。
OBJECTIVE: To observe the expression of fibroblast growth factor 23 (FGF23) and calcium and phosphorus metabolism in mouse models of chronic kidney disease (CKD) induced by 5/6 nephrectomy (5/6 Nx) in order to study the relationship between mineral and bone metabolism The pathogenesis of disorders. Methods C57 male mice were reared for 1 week. About 5/6 left kidneys were removed from the model group. Right kidney was excised one week later and hematuria and kidney tissue samples were collected after 16 weeks. The hematuria FGF23 and parathyroid (PTH), active vitamin D, calcium, phosphorus and other indicators to observe the renal pathology. Results Compared with the sham operation group and the normal control group, the body weight decreased, the serum FGF23, PTH, phosphorus and other abnormalities increased at the 16th week after operation, the level of active vitamin D decreased significantly and the urinary protein increased. Kidney pathology found mesangial matrix hyperplasia, collagen deposition and fibrosis significantly, and glomerular compensatory hypertrophy. Conclusion 5/6 Nx mice can show obvious characteristics of mineral and bone metabolism disorder, which is a suitable model for studying CKD osteopathy.